GPBAR1 Polyclonal antibody
GPBAR1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
26739-1-AP targets GPBAR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | GPBAR1 fusion protein Ag24893 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | G protein-coupled bile acid receptor 1 |
| Synonyms | BG37, GPBAR1, GPCR, GPCR19, GPR131, hBG37, hGPCR19, M BAR, TGR5 |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC033625 |
| Gene Symbol | GPBAR1 |
| Gene ID (NCBI) | 151306 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDU6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPBAR1 (G-protein coupled bile acid receptor 1), also known as TGR5 or M-BAR, is a cell-surface receptor for bile acid which belongs to the G-protein coupled receptor 1 family (20236244). GPBAR1 gene expression is widely distributed, including endocrine glands, adipocytes, muscles, immune organs, spinal cord, and the enteric nervous system (24411485, 20665558, 22521118). GPBAR1 activation has some important biological effects such as anti-inflammatory, energy homeostasis and metabolism, anti-apoptotic, and choleretic functions (24411485, 22521118). In addition, GPBAR1 is related to several diseases, such as Metabolic and cardiovascular disorders, Hepatic and pancreatic disorders, Inflammatory bowel diseases, Gastrointestinal cancer, and so on (24411485).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPBAR1 antibody 26739-1-AP | Download protocol |
| IHC protocol for GPBAR1 antibody 26739-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Ginsenoside Rh4 alleviates gastrointestinal mucositis and enhances chemotherapy efficacy through modulating gut microbiota | ||
Int Immunopharmacol Targeting TGR5 to mitigate liver fibrosis: Inhibition of hepatic stellate cell activation through modulation of mitochondrial fission |