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Species Reactivity
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Species Reactivity
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验证数据展示

  • WB analysis using 13521-1-AP

    WB analysis using 13521-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13521-1-AP (Glypican 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Glypican 2 Polyclonal antibody

Glypican 2 Polyclonal Antibody for WB, ELISA
Cat No. 13521-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Glypican 2, GPC2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, unboiled mouse brain tissue, unboiled rat brain tissue, T-47D cells, mouse spleen tissue, mouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13521-1-AP targets Glypican 2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Glypican 2 fusion protein Ag4041 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glypican 2
Synonyms Glypican 2, GPC2
Calculated Molecular Weight 579 aa, 63 kDa
Observed Molecular Weight62，70-100 kDa
GenBank Accession NumberBC027972
Gene Symbol GPC2
Gene ID (NCBI) 221914
RRIDAB_3085417
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N158
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glypican 2 (GPC2) is a heparan sulfate proteoglycan that is linked to the outer leaflet of the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage(PMID: 18505598). It is expected to be located in cell membrane and highly overexpressed in a high percentage of human neuroblastomas, medulloblastomas, and retinoblastomas. The calculated molecular weight of GPC2 is 62 kDa. And other bands are the result of glycosylation modification. Recent studies demonstrate that expression of the GPC2 protein is significantly increased in neuroblastoma and undetectable in normal tissues, including brain, heart, lung, and kidney, indicating that GPC2 is a suitable tumor antigen in neuroblastoma (PMID: 30352677).

实验方案

Product Specific Protocols
WB protocol for Glypican 2 antibody 13521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols