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Glypican 2 Polyclonal antibody

Glypican 2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27594-1-AP

Print datasheet

Synonyms

GPC2, Secreted glypican-2, FLJ38962

验证数据展示

  • WB analysis using 27594-1-AP

    WB analysis using 27594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27594-1-AP (GPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inunboiled mouse brain tissue, unboiled rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27594-1-AP targets Glypican 2 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Glypican 2 fusion protein Ag26734 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glypican 2
Synonyms GPC2, Secreted glypican-2, FLJ38962
Calculated Molecular Weight 579 aa, 63 kDa
Observed Molecular Weight62-67 kDa
GenBank Accession NumberBC027972
Gene Symbol GPC2
Gene ID (NCBI) 221914
RRIDAB_3085972
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N158
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPC2 (glypican 2), which is expected to be located in cell membrane and extracellular space. It is expressed in lymphoid tissue, skin and testis. The calculated molecular weight of the protein is 62 kDa. GPC2 is mainly active in growing nervous tissues and thyroid cancer tissues (PMID: 28616017). It participates in the growth and differentiation of neuronal axons. Increasing evidence has demonstrated the overexpression of GPC2 in neuroblastoma, a kind of childhood cancer. There is a discovery that GPC2 can be employed as a diagnostic, prognostic, and immunological predictor of generalized cancers. The study may broaden the train of thought toward application of GPC2 in immunotherapy (PMID: 35345673).

实验方案

Product Specific Protocols
WB protocol for Glypican 2 antibody 27594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols