验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, rat brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13048-1-AP targets Glypican 4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Glypican 4 fusion protein Ag3670 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glypican 4 |
| 别名 | GPC4, Glypican 4/6, Secreted glypican-4, K-glypican, K glypican |
| 计算分子量 | 556 aa, 62 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | BC017166 |
| 基因名称 | Glypican 4 |
| Gene ID (NCBI) | 2239 |
| RRID | AB_10640157 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75487 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation (PMID: 9931407). In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus (PMID: 10585884). They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Human GPC4 is a 556-amino acid protein, and shows 93.5% identity to mouse K-glypican. The gene of GPC4 has been mapped to Xq26, and its mRNA is widely expressed in human tissues, including lung, kidney, heart, placenta, skeletal muscle, and pancreas. GPC4 has also been shown to be present in astrocytes, haematopoietic-progenitor and bone-marrow-stromal cells. Studies suggest that GPC4 is involved in the regulation of Wnt signaling and astrocyte GPC4 plays a role in formation of excitatory synapses (PMID: 22302992; 22722203). This antobody can recognize both full length (65 kDa) and cleaved (35 kDa) GPC4 as well as GPC6 (PMID: 18055828; 22722203).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Glypican 4 antibody 13048-1-AP | Download protocol |
| IHC protocol for Glypican 4 antibody 13048-1-AP | Download protocol |
| IF protocol for Glypican 4 antibody 13048-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Astrocyte glypicans 4 and 6 promote formation of excitatory synapses via GluA1 AMPA receptors.
| ||
Neuron Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation. | ||
Sci Adv Cocaine preference and neuroadaptations are maintained by astrocytic NMDA receptors in the nucleus accumbens. | ||
Nat Commun Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.
| ||
Nat Commun CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis. |