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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 13451-1-AP (same clone as 13451-1-PBS)

    WB analysis using 13451-1-AP (same clone as 13451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13451-1-AP (GPD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13451-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 13451-1-AP (same clone as 13451-1-PBS)

    IF Staining of HepG2 using 13451-1-AP (same clone as 13451-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13451-1-AP (GPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13451-1-PBS in a different storage buffer formulation.

GPD1 Polyclonal antibody, PBS Only

GPD1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 13451-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

GPDC, GPD C, GPD 1, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, EC:1.1.1.8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GPD1 Ready-To-Use IHC Kit. GPD1 primary antibody included.

产品信息

13451-1-PBS targets GPD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GPD1 fusion protein Ag4278 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycerol-3-phosphate dehydrogenase 1 (soluble)
别名 GPDC, GPD C, GPD 1, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, EC:1.1.1.8
计算分子量 349 aa, 38 kDa
观测分子量 32-42 kDa
GenBank蛋白编号BC032234
基因名称 GPD1
Gene ID (NCBI) 2819
RRIDAB_10646471
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21695
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPD1 (Glycerol-3-phosphate dehydrogenase 1) is an important enzyme belonging to the NAD-dependent glycerol-3-phosphate dehydrogenase family. Its C-terminal structural domain contains multiple helical structures for binding the substrate DHAP, and its N-terminal structural domain contains a β-folded core for binding NADH. GPD1 catalyzes the conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotinamide adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+, and plays a key role in carbohydrate and lipid metabolism. GPD1 also works with mitochondrial glycerol-3-phosphate dehydrogenase to form a glycerophosphate shuttle system that facilitates the transfer of reducing equivalents from the cytoplasm to the mitochondria. Abnormal activity of GPD1 has been associated with a variety of metabolic disorders, such as obesity, hypertriglyceridemia, and GPD1 has been implicated in cancer, potentially acting as a tumor suppressor.