验证数据展示
产品信息
84800-1-PBS targets GPD1 as part of a matched antibody pair:
MP01579-1: 84800-1-PBS capture and 84800-2-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Applications | WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | GPD1 fusion protein Ag4278 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | glycerol-3-phosphate dehydrogenase 1 (soluble) |
| Synonyms | GPD C, GPD 1, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, EC:1.1.1.8, cGPDH |
| Calculated Molecular Weight | 349 aa, 38 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC032234 |
| Gene Symbol | GPD1 |
| Gene ID (NCBI) | 2819 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P21695 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
GPD1 (Glycerol-3-phosphate dehydrogenase 1) is an important enzyme belonging to the NAD-dependent glycerol-3-phosphate dehydrogenase family. Its C-terminal structural domain contains multiple helical structures for binding the substrate DHAP, and its N-terminal structural domain contains a β-folded core for binding NADH. GPD1 catalyzes the conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotinamide adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+, and plays a key role in carbohydrate and lipid metabolism. GPD1 also works with mitochondrial glycerol-3-phosphate dehydrogenase to form a glycerophosphate shuttle system that facilitates the transfer of reducing equivalents from the cytoplasm to the mitochondria. Abnormal activity of GPD1 has been associated with a variety of metabolic disorders, such as obesity, hypertriglyceridemia, and GPD1 has been implicated in cancer, potentially acting as a tumor suppressor.