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验证数据展示

  • WB analysis using 84800-4-RR

    WB analysis using 84800-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84800-4-RR (GPD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

GPD1 Recombinant antibody

GPD1 Recombinant Antibody for WB, ELISA
Cat No. 84800-4-RR

Print datasheet

CloneNo. 242359C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

GPD C, GPD 1, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, EC:1.1.1.8, cGPDH

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, mouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy GPD1 Ready-To-Use IHC Kit. GPD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84800-4-RR targets GPD1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GPD1 fusion protein Ag4278 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glycerol-3-phosphate dehydrogenase 1 (soluble)
Synonyms GPD C, GPD 1, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, EC:1.1.1.8, cGPDH
Calculated Molecular Weight 349 aa, 38 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC032234
Gene Symbol GPD1
Gene ID (NCBI) 2819
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21695
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPD1 (Glycerol-3-phosphate dehydrogenase 1) is an important enzyme belonging to the NAD-dependent glycerol-3-phosphate dehydrogenase family. Its C-terminal structural domain contains multiple helical structures for binding the substrate DHAP, and its N-terminal structural domain contains a β-folded core for binding NADH. GPD1 catalyzes the conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotinamide adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+, and plays a key role in carbohydrate and lipid metabolism. GPD1 also works with mitochondrial glycerol-3-phosphate dehydrogenase to form a glycerophosphate shuttle system that facilitates the transfer of reducing equivalents from the cytoplasm to the mitochondria. Abnormal activity of GPD1 has been associated with a variety of metabolic disorders, such as obesity, hypertriglyceridemia, and GPD1 has been implicated in cancer, potentially acting as a tumor suppressor.

实验方案

Product Specific Protocols
WB protocol for GPD1 antibody 84800-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols