gephyrin Polyclonal antibody
gephyrin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Jurkat cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
IF | See 2 publications below |
产品信息
12681-1-AP targets gephyrin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse, rat |
Immunogen | gephyrin fusion protein Ag3370 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | gephyrin |
Synonyms | GPHN, GPHRYN, GPH, GEPH, Domain G |
Calculated Molecular Weight | 769 aa, 83 kDa |
Observed Molecular Weight | 93 kDa |
GenBank Accession Number | BC030016 |
Gene Symbol | GPHN |
Gene ID (NCBI) | 10243 |
RRID | AB_2247770 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NQX3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for gephyrin antibody 12681-1-AP | Download protocol |
IHC protocol for gephyrin antibody 12681-1-AP | Download protocol |
IF protocol for gephyrin antibody 12681-1-AP | Download protocol |
IP protocol for gephyrin antibody 12681-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Neurosci Microglial Tmem59 Deficiency Impairs Phagocytosis of Synapse and Leads to Autism-Like Behaviors in Mice. | ||
Cell Rep Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia. | ||
Neurosci Lett Microglia participate in postoperative cognitive dysfunction by mediating the loss of inhibitory synapse through the complement pathway | ||
Arthritis Rheumatol Anti-Gephyrin antibodies: A novel specificity in patients with systemic sclerosis and lower bowel dysfunction | ||
Adv Funct Mater Metabolically Glycoengineered Neural Stem Cells Boost Neural Repair After Cardiac Arrest |