gephyrin Polyclonal antibody

gephyrin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12681-1-AP

Print datasheet

Synonyms

GPHN, GPHRYN, GPH, GEPH, Domain G



经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12681-1-AP targets gephyrin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen gephyrin fusion protein Ag3370 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gephyrin
Synonyms GPHN, GPHRYN, GPH, GEPH, Domain G
Calculated Molecular Weight 769 aa, 83 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC030016
Gene Symbol GPHN
Gene ID (NCBI) 10243
RRIDAB_2247770
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.

实验方案

Product Specific Protocols
WB protocol for gephyrin antibody 12681-1-APDownload protocol
IHC protocol for gephyrin antibody 12681-1-APDownload protocol
IF protocol for gephyrin antibody 12681-1-APDownload protocol
IP protocol for gephyrin antibody 12681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Neurosci

Microglial Tmem59 Deficiency Impairs Phagocytosis of Synapse and Leads to Autism-Like Behaviors in Mice.

Authors - Jian Meng
mouseWB

Cell Rep

Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia.

Authors - Giuseppe Sciamanna
mouseWB

Neurosci Lett

Microglia participate in postoperative cognitive dysfunction by mediating the loss of inhibitory synapse through the complement pathway

Authors - Xiaoxiang Tan
mouseWB

Synapse

Triptolide injection reduces Alzheimer's disease-like pathology in mice

Authors - Rui Mao
mouseIF

Arthritis Rheumatol

Anti-Gephyrin antibodies: A novel specificity in patients with systemic sclerosis and lower bowel dysfunction

Authors - Zsuzsanna H McMahan
ratIF

Adv Funct Mater

Metabolically Glycoengineered Neural Stem Cells Boost Neural Repair After Cardiac Arrest

Authors - Jian Du