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  • Featured Product
  • KD/KO Validated

GPI Monoclonal antibody, PBS Only

GPI Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11E4

Cat no : 67178-1-PBS

Print datasheet

Synonyms

AMF, Autocrine motility factor, Glucose 6 phosphate isomerase, glucose phosphate isomerase, GNPI, GPI, Neuroleukin, NLK, PGI, PHI, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36

验证数据展示

  • WB analysis using 67178-1-Ig (same clone as 67178-1-PBS)

    WB analysis using 67178-1-Ig (same clone as 67178-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67178-1-Ig (GPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67178-1-Ig (same clone as 67178-1-PBS)

    WB analysis of HeLa using 67178-1-Ig (same clone as 67178-1-PBS)

    WB result of GPI antibody (67178-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

    IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

    IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 67178-1-Ig (same clone as 67178-1-PBS)

    IF Staining of PC-3 using 67178-1-Ig (same clone as 67178-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using 67178-1-Ig (GPI antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

产品信息

67178-1-PBS targets GPI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen GPI fusion protein Ag7419 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glucose phosphate isomerase
Synonyms AMF, Autocrine motility factor, Glucose 6 phosphate isomerase, glucose phosphate isomerase, GNPI, GPI, Neuroleukin, NLK, PGI, PHI, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 55-64 kDa
GenBank Accession NumberBC004982
Gene Symbol GPI
Gene ID (NCBI) 2821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06744
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).