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验证数据展示

  • IHC staining of human breast cancer using 14728-1-AP

    IHC staining of human breast cancer using 14728-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14728-1-AP (GPKOW antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 14728-1-AP

    IF Staining of HeLa using 14728-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14728-1-AP (GPKOW antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HeLa using 14728-1-AP

    WB analysis of HeLa using 14728-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14728-1-AP (GPKOW antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 14728-1-AP

    WB analysis using 14728-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14728-1-AP (GPKOW antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

GPKOW Polyclonal antibody

GPKOW Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14728-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

T54, Protein T54, GPATCH5, G-patch domain-containing protein 5, G-patch domain and KOW motifs-containing protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, human liver tissue, HeLa cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14728-1-AP targets GPKOW in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GPKOW fusion protein Ag6487 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G patch domain and KOW motifs
别名 T54, Protein T54, GPATCH5, G-patch domain-containing protein 5, G-patch domain and KOW motifs-containing protein
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC000397
基因名称 GPKOW
Gene ID (NCBI) 27238
RRIDAB_2112797
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92917
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPKOW antibody 14728-1-APDownload protocol
IHC protocol for GPKOW antibody 14728-1-APDownload protocol
IF protocol for GPKOW antibody 14728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols