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验证数据展示

  • WB analysis using 68338-1-Ig

    WB analysis using 68338-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68338-1-Ig (GPKOW antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

GPKOW Monoclonal antibody

GPKOW Monoclonal Antibody for WB, ELISA
Cat No. 68338-1-Ig

产品说明书

CloneNo. 1G11C4

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

G patch domain and KOW motifs, GPATC5, GPATCH5, GPKOW, Protein MOS2 homolog, Protein T54, T54

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, LNcaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68338-1-Ig targets GPKOW in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 GPKOW fusion protein Ag6545 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 G patch domain and KOW motifs
别名 G patch domain and KOW motifs, GPATC5, GPATCH5, GPKOW, Protein MOS2 homolog, Protein T54, T54
计算分子量 52 kDa
观测分子量52 kDa
GenBank蛋白编号BC000397
基因名称 GPKOW
Gene ID (NCBI) 27238
RRIDAB_3085062
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ92917
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPKOW is a RNA-binding protein involved in pre-mRNA splicing.It interacts directly with protein kinase A and protein kinase X and is also found associated with the spliceosome.

实验方案

Product Specific Protocols
WB protocol for GPKOW antibody 68338-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols