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GPNMB Polyclonal antibody

GPNMB Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 27037-1-AP

Print datasheet

Synonyms

GPNMB, HGFIN, Transmembrane glycoprotein NMB

验证数据展示

  • WB analysis using 27037-1-AP

    WB analysis using 27037-1-AP

    TT cells were subjected to SDS PAGE followed by western blot with 27037-1-AP (GPNMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inTT cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27037-1-AP targets GPNMB in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen GPNMB fusion protein Ag25826 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycoprotein (transmembrane) nmb
Synonyms GPNMB, HGFIN, Transmembrane glycoprotein NMB
Calculated Molecular Weight 64 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC011595
Gene Symbol GPNMB
Gene ID (NCBI) 10457
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14956
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

实验方案

Product Specific Protocols
WB protocol for GPNMB antibody 27037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols