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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66926-1-Ig (same clone as 66926-1-PBS)

    WB analysis using 66926-1-Ig (same clone as 66926-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

  • WB analysis using 66926-1-Ig (same clone as 66926-1-PBS)

    WB analysis using 66926-1-Ig (same clone as 66926-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

    IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GPNMB antibody included
  • IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

    IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GPNMB antibody included
  • IF Staining of HeLa using 66926-1-Ig (same clone as 66926-1-PBS)

    IF Staining of HeLa using 66926-1-Ig (same clone as 66926-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (66926-1-Ig, Clone: 2B10B8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

GPNMB Monoclonal antibody, PBS Only

GPNMB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66926-1-PBS

产品说明书

CloneNo. 2B10B8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GPNMB Ready-To-Use IHC Kit. GPNMB primary antibody included.

产品信息

66926-1-PBS targets GPNMB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GPNMB fusion protein Ag26747 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glycoprotein (transmembrane) nmb
别名 UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type
计算分子量 64 kDa
观测分子量 64-70 kDa
GenBank蛋白编号BC011595
基因名称 GPNMB
Gene ID (NCBI) 10457
RRIDAB_2882253
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14956
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.