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GPNMB Monoclonal antibody, PBS Only

GPNMB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B10B8

Cat No : 66926-1-PBS

Print datasheet

Synonyms

GPNMB, HGFIN, Transmembrane glycoprotein NMB

验证数据展示

  • WB analysis using 66926-1-Ig (same clone as 66926-1-PBS)

    WB analysis using 66926-1-Ig (same clone as 66926-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

    IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

    IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66926-1-Ig (same clone as 66926-1-PBS)

    IF Staining of HeLa using 66926-1-Ig (same clone as 66926-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (66926-1-Ig, Clone: 2B10B8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

产品信息

66926-1-PBS targets GPNMB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen GPNMB fusion protein Ag26747 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycoprotein (transmembrane) nmb
Synonyms GPNMB, HGFIN, Transmembrane glycoprotein NMB
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 64-70 kDa
GenBank Accession NumberBC011595
Gene Symbol GPNMB
Gene ID (NCBI) 10457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14956
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.