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GPNMB Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG2a

Reactivity

Capture: Human

Detection: Human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 15.6-1000 pg/mL

Cat No : MP50045-1

Print datasheet

Synonyms

GPNMB, HGFIN, Transmembrane glycoprotein NMB

Validation Data Gallery

  • Sandwich ELISA standard curve of MP50045-1, GPNMB Monoclonal Matched Antibody Pair - PBS only. 66926-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag26747. 66926-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

    Sandwich ELISA standard curve of MP50045-1

    Sandwich ELISA standard curve of MP50045-1, GPNMB Monoclonal Matched Antibody Pair - PBS only. 66926-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag26747. 66926-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP50045-1 targets GPNMB in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: GPNMB Monoclonal antibody, PBS Only (Capture) 66926-2-PBS (2H3E12). 100 μg. Concentration 1 mgl/ml.

Detection antibody: GPNMB Monoclonal antibody, PBS Only (Detector) 66926-4-PBS (1E5B9). 100 μg. Concentration 1 mgl/ml.

Alternative GPNMB matched antibody pairs: MP50045-2

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 66926-2-PBS
Tested Reactivity Human
Immunogen GPNMB fusion protein Ag26747
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.03 x 10-10M
KOff=1.53 x 10-6M
KOn=1.49 x 104M
Full Name glycoprotein (transmembrane) nmb
Synonyms GPNMB, HGFIN, Transmembrane glycoprotein NMB
GenBank accession numberBC011595
Gene symbol GPNMB
UniprotQ14956
Gene ID (NCBI) 10457
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 66926-4-PBS
Tested Reactivity Human
Immunogen GPNMB fusion protein Ag26747
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Affinity
KD=8.73 x 10-11M
KOff=1.93 x 10-6M
KOn=2.21 x 104M
Full Name glycoprotein (transmembrane) nmb
Synonyms GPNMB, HGFIN, Transmembrane glycoprotein NMB
GenBank accession numberBC011595
Gene symbol GPNMB
UniprotQ14956
Gene ID (NCBI) 10457
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.