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GPR108 Polyclonal antibody

GPR108 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24009-1-AP

Print datasheet

Synonyms

G protein coupled receptor 108, GPR108, LUSTR2, Protein GPR108

验证数据展示

  • WB analysis of mouse kidney using 24009-1-AP

    WB analysis of mouse kidney using 24009-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24009-1-AP (GPR108 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 24009-1-AP

    IHC staining of mouse brain using 24009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24009-1-AP (GPR108 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 24009-1-AP

    IHC staining of human kidney using 24009-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24009-1-AP (GPR108 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 24009-1-AP

    IHC staining of human kidney using 24009-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24009-1-AP (GPR108 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24009-1-AP targets GPR108 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GPR108 fusion protein Ag20887 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 108
Synonyms G protein coupled receptor 108, GPR108, LUSTR2, Protein GPR108
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61-70 kDa
GenBank Accession NumberBC007862
Gene Symbol GPR108
Gene ID (NCBI) 56927
RRIDAB_2879401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9NPR9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR108 is a seven-transmembrane family protein that falls in the GPCRs superfamily (PMID:30332431). GPR108 was identified as a highly conserved AAV entry factor implicated in cellular immune responses to AAV (PMID:31784416). Of note is its role in activating nuclear factor kappa-B (NF-κB) signaling, which implied the potential involvement of GPR108 in inflammation-related diseases as well as cancers through dysregulating NF-κB activity (PMID:35659621).

实验方案

Product Specific Protocols
WB protocol for GPR108 antibody 24009-1-APDownload protocol
IHC protocol for GPR108 antibody 24009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

J Virol

Interplay between Furin and Sialoglycans in Modulating Adeno-Associated Viral Cell Entry

Authors - Timothy J Smith
View Article
humanWB

Nat Chem Biol

Chemoproteomics reveals microbiota-derived aromatic monoamine agonists for GPRC5A

Authors - Xiaohui Zhao
View Article