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验证数据展示

  • IF Staining of RAW 264.7 using 31781-1-AP

    IF Staining of RAW 264.7 using 31781-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using GPR112 antibody (31781-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IHC staining of mouse colon using 31781-1-AP

    IHC staining of mouse colon using 31781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31781-1-AP (GPR112 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 31781-1-AP

    IHC staining of mouse colon using 31781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31781-1-AP (GPR112 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR112 Polyclonal antibody

GPR112 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 31781-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

RP1 299I16, PGR17, G protein coupled receptor 112

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31781-1-AP targets GPR112 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GPR112 fusion protein Ag36114 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 112
别名 RP1 299I16, PGR17, G protein coupled receptor 112
计算分子量333kDa
GenBank蛋白编号NM_153834
基因名称 GPR112
Gene ID (NCBI) 139378
RRIDAB_3670112
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8IZF6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR112 is a member of the adhesion G protein-coupled receptor (aGPCR) family. These receptors are characterized by a complex architecture and large size, mainly attributed to their N terminus, which contains a variety of domains involved in cell-cell and cell-matrix interactions. The function of GPR112 and other aGPCRs is closely related to key aspects of the development, architecture, and function of the nervous system. They are involved in neuronal migration, axon guidance, dendritogenesis, and synaptogenesis. Additionally, aGPCRs, including GPR112, have been implicated in the development of myelinating glial cells, which are crucial for proper neuronal signal propagation.

实验方案

Product Specific Protocols
IHC protocol for GPR112 antibody 31781-1-APDownload protocol
IF protocol for GPR112 antibody 31781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols