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验证数据展示

  • WB analysis of HEK-293 using 14047-1-AP

    WB analysis of HEK-293 using 14047-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14047-1-AP (GPR116 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human kidney using 14047-1-AP

    IHC staining of human kidney using 14047-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14047-1-AP (GPR116 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 14047-1-AP

    IHC staining of human kidney using 14047-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14047-1-AP (GPR116 Antibody) at dilution of 1:100 (under 40x lens).

GPR116 Polyclonal antibody

GPR116 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14047-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KPG_001, KIAA0758, G-protein coupled receptor 116, G protein coupled receptor 116, Adhesion G protein-coupled receptor F5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Y79 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14047-1-AP targets GPR116 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 GPR116 fusion protein Ag5214 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 116
别名 KPG_001, KIAA0758, G-protein coupled receptor 116, G protein coupled receptor 116, Adhesion G protein-coupled receptor F5
计算分子量 1346 aa, 149 kDa
观测分子量 149 kDa
GenBank蛋白编号BC066121
基因名称 GPR116
Gene ID (NCBI) 221395
RRIDAB_2113095
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IZF2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR116 (ADGRF5, adhesion G protein-coupled receptor F5) is involved in the G protein-coupled receptor signaling pathway and cell surface receptor signaling pathway. may act upstream of or within several processes, including glomerular filtration; pharyngeal arch artery morphogenesis; and surfactant homeostasis.

实验方案

Product Specific Protocols
WB protocol for GPR116 antibody 14047-1-APDownload protocol
IHC protocol for GPR116 antibody 14047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Rep Med

Comprehensive proteogenomic characterization of rare kidney tumors

Authors - Ginny Xiaohe Li
View Article
humanWB,IHC

Oncotarget

High expression of GPR116 indicates poor survival outcome and promotes tumor progression in colorectal carcinoma.

Authors - Li Yang
View Article
humanIHC

Medicine (Baltimore)

GPR116 overexpression correlates with poor prognosis in gastric cancer.

Authors - Tian Zheng
View Article