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GPR116 Polyclonal antibody

GPR116 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14047-1-AP

Print datasheet

Synonyms

KPG_001, KIAA0758, G-protein coupled receptor 116, G protein coupled receptor 116, Adhesion G protein-coupled receptor F5

验证数据展示

  • WB analysis of HEK-293 using 14047-1-AP

    WB analysis of HEK-293 using 14047-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14047-1-AP (GPR116 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human kidney using 14047-1-AP

    IHC staining of human kidney using 14047-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14047-1-AP (GPR116 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 14047-1-AP

    IHC staining of human kidney using 14047-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14047-1-AP (GPR116 Antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Y79 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14047-1-AP targets GPR116 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen GPR116 fusion protein Ag5214 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 116
Synonyms KPG_001, KIAA0758, G-protein coupled receptor 116, G protein coupled receptor 116, Adhesion G protein-coupled receptor F5
Calculated Molecular Weight 1346 aa, 149 kDa
Observed Molecular Weight 149 kDa
GenBank Accession NumberBC066121
Gene Symbol GPR116
Gene ID (NCBI) 221395
RRIDAB_2113095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR116 (ADGRF5, adhesion G protein-coupled receptor F5) is involved in the G protein-coupled receptor signaling pathway and cell surface receptor signaling pathway. may act upstream of or within several processes, including glomerular filtration; pharyngeal arch artery morphogenesis; and surfactant homeostasis.

实验方案

Product Specific Protocols
WB protocol for GPR116 antibody 14047-1-APDownload protocol
IHC protocol for GPR116 antibody 14047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Rep Med

Comprehensive proteogenomic characterization of rare kidney tumors

Authors - Ginny Xiaohe Li
View Article
humanWB,IHC

Oncotarget

High expression of GPR116 indicates poor survival outcome and promotes tumor progression in colorectal carcinoma.

Authors - Li Yang
View Article
humanIHC

Medicine (Baltimore)

GPR116 overexpression correlates with poor prognosis in gastric cancer.

Authors - Tian Zheng
View Article