验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, MDA-MB-231 cells, Calu-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66967-2-Ig targets GPR116 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | GPR116 fusion protein Ag5820 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 116 |
| 别名 | KIAA0758, G-protein coupled receptor 116, G protein coupled receptor 116, Adhesion G protein-coupled receptor F5, ADGRF5 |
| 计算分子量 | 1346 aa, 149 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC066121 |
| 基因名称 | GPR116 |
| Gene ID (NCBI) | 221395 |
| RRID | AB_3670377 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8IZF2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR116 (ADGRF5, adhesion G protein-coupled receptor F5) is involved in the G protein-coupled receptor signaling pathway and cell surface receptor signaling pathway. may act upstream of or within several processes, including glomerular filtration; pharyngeal arch artery morphogenesis; and surfactant homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR116 antibody 66967-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |