验证数据展示
经过测试的应用
| Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11929-1-AP targets GPR137 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GPR137 fusion protein Ag2611 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 137 |
| 别名 | C11orf4, G protein coupled receptor 137, GPR137, GPR137A, TM7SF1L1 |
| 计算分子量 | 396 aa, 44 kDa |
| GenBank蛋白编号 | BC033920 |
| 基因名称 | GPR137 |
| Gene ID (NCBI) | 56834 |
| RRID | AB_10694423 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96N19 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
G protein-coupled receptors (GPRs) are highly related to oncogenesis and cancer metastasis. G protein-coupled receptor 137 (GPR137) was initially reported as a novel orphan GPR about 10 years ago. GPR137 is ubiquitously expressed in the central nervous system (CNS), mainly in the hippocampus (PMID: 22025619). GPR137 is involved in the progression of human glioma, suggesting GPR137 as a potential oncogene of glioma cells (PMID: 24870220). Recent study shows that GPR137 plays an important role in colon cancer cell proliferation (PMID: 25301753).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPR137 antibody 11929-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biotechnol Appl Biochem Knockdown of GPR137 by RNAi inhibits pancreatic cancer cell growth and induces apoptosis. | ||
Biotechnol Appl Biochem G-protein-coupled receptor 137 accelerates proliferation of urinary bladder cancer cells in vitro.
| ||
Cell Biol Int Down-Regulation of G Protein-Coupled Receptor 137 by RNA Interference Inhibits Cell Growth of Two Hepatoma Cell Lines. | ||
Neurol Sci Inhibition of GPR137 expression reduces the proliferation and colony formation of malignant glioma cells. | ||
J Bone Oncol Small interfering RNA-mediated silencing of G-protein-coupled receptor 137 inhibits growth of osteosarcoma cells. | ||
Mol Med Rep RNA interference-mediated silencing of G protein-coupled receptor 137 inhibits human gastric cancer cell growth.
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