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验证数据展示

  • IHC staining of human kidney using 28096-1-AP

    IHC staining of human kidney using 28096-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28096-1-AP (GPR141 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 28096-1-AP

    IHC staining of human kidney using 28096-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28096-1-AP (GPR141 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28096-1-AP

    IHC staining of mouse brain using 28096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28096-1-AP (GPR141 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR141 Polyclonal antibody

GPR141 Polyclonal Antibody for IHC, ELISA
Cat No. 28096-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, ELISA

Synonyms

G protein coupled receptor 141, GPR141, PGR13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28096-1-AP targets GPR141 in IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen GPR141 fusion protein Ag23129 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 141
Synonyms G protein coupled receptor 141, GPR141, PGR13
Calculated Molecular Weight 305 aa, 35 kDa
GenBank Accession NumberBC120951
Gene Symbol GPR141
Gene ID (NCBI) 353345
RRIDAB_3086028
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z602
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR141 (G-protein-coupled receptor 141) is a class A orphan receptor molecule of the rhodopsin family. Increased GPR141 expression enhances the migratory behavior of breast cancer, driving oncogenic pathways both in vitro and in vivo through activation of epithelial to mesenchymal transition (EMT), oncogenic mediators, and regulation of p-mTOR/p53 signaling (PMID: 37204251).

实验方案

Product Specific Protocols
IHC protocol for GPR141 antibody 28096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols