验证数据展示
经过测试的应用
Positive IHC detected in | human kidney tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28096-1-AP targets GPR141 in IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | IHC, ELISA Application Description |
Tested Reactivity | Human, mouse |
Immunogen | GPR141 fusion protein Ag23129 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | G protein-coupled receptor 141 |
Synonyms | G protein coupled receptor 141, GPR141, PGR13 |
Calculated Molecular Weight | 305 aa, 35 kDa |
GenBank Accession Number | BC120951 |
Gene Symbol | GPR141 |
Gene ID (NCBI) | 353345 |
RRID | AB_3086028 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q7Z602 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR141 (G-protein-coupled receptor 141) is a class A orphan receptor molecule of the rhodopsin family. Increased GPR141 expression enhances the migratory behavior of breast cancer, driving oncogenic pathways both in vitro and in vivo through activation of epithelial to mesenchymal transition (EMT), oncogenic mediators, and regulation of p-mTOR/p53 signaling (PMID: 37204251).
实验方案
Product Specific Protocols | |
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IHC protocol for GPR141 antibody 28096-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |