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验证数据展示

  • WB analysis using 30104-1-AP

    WB analysis using 30104-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30104-1-AP (GPR151 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

GPR151 Polyclonal antibody

GPR151 Polyclonal Antibody for WB, ELISA
Cat No. 30104-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

G protein coupled receptor 151, GALR4, GALRL, GPCR, GPCR 2037, GPR151, PGR7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30104-1-AP targets GPR151 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen GPR151 fusion protein Ag32442 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 151
Synonyms G protein coupled receptor 151, GALR4, GALRL, GPCR, GPCR 2037, GPR151, PGR7
Calculated Molecular Weight47kd
Observed Molecular Weight46 kDa
GenBank Accession NumberNM_194251
Gene Symbol GPR151
Gene ID (NCBI) 134391
RRIDAB_3086229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDV0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G protein-coupled receptors (GPCRs) are a superfamily of cell-surface receptors which are important for intracellular signal transduction. GPR151 (G protein-coupled receptor 151) is highly enriched in the the medial and lateral habenula and is expressed at presynaptic membranes and synaptic vesicles (PMID: 32098843). GPR151 has been reported to play an important role in modulating neuropathic pain and controling nicotine addiction vulnerability (PMID: 32098843,34244727,30373770).

实验方案

Product Specific Protocols
WB protocol for GPR151 antibody 30104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols