验证数据展示
经过测试的应用
| Positive WB detected in | U-87 MG cells, mouse brain tissue, HEK-293 cells, rat brain tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human ovary tissue, mouse liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IHC | See 4 publications below |
产品信息
17950-1-AP targets GPR177 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, goat |
| 免疫原 | GPR177 fusion protein Ag12428 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 177 |
| 别名 | C1orf139, EVI, G protein coupled receptor 177, GPR177, MRP, Protein wntless homolog, Sprinter, WLS, Wntless |
| 计算分子量 | 541 aa, 62 kDa |
| 观测分子量 | 46-65 kDa |
| GenBank蛋白编号 | BC110826 |
| 基因名称 | GPR177 |
| Gene ID (NCBI) | 79971 |
| RRID | AB_2034928 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5T9L3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR177 antibody 17950-1-AP | Download protocol |
| IHC protocol for GPR177 antibody 17950-1-AP | Download protocol |
| IP protocol for GPR177 antibody 17950-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs. | ||
Front Physiol A Longitudinal Study of T2 Mapping Combined With Diffusion Tensor Imaging to Quantitatively Evaluate Tissue Repair of Rat Skeletal Muscle After Frostbite. | ||
Onco Targets Ther Wntless (Wls): A Prognostic Index for Progression and Patient Survival of Breast Cancer. | ||
Int Ophthalmol The effect of Wnt/β-catenin pathway on the scleral remolding in the mouse during form deprivation. | ||
Cells Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat. |
