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验证数据展示

  • FC experiment of SH-SY5Y using 19951-1-AP

    FC experiment of SH-SY5Y using 19951-1-AP

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug GPR22 Polyclonal antibody (19951-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of mouse heart using 19951-1-AP

    WB analysis of mouse heart using 19951-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19951-1-AP (GPR22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 19951-1-AP

    IHC staining of human testis using 19951-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19951-1-AP (GPR22 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 19951-1-AP

    IHC staining of human testis using 19951-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19951-1-AP (GPR22 Antibody) at dilution of 1:100 (under 40x lens).

GPR22 Polyclonal antibody

GPR22 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19951-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

G-protein coupled receptor 22, G protein coupled receptor 22

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19951-1-AP targets GPR22 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 22
别名 G-protein coupled receptor 22, G protein coupled receptor 22
计算分子量 49 kDa
观测分子量 49 kDa
GenBank蛋白编号NM_005295
基因名称 GPR22
Gene ID (NCBI) 2845
RRIDAB_10644129
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99680
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR22 Belongs to the G-protein coupled receptor 1 family. GPR22 is an Orphan receptor. The antibody recognize the C-term of GPR22.

实验方案

Product Specific Protocols
WB protocol for GPR22 antibody 19951-1-APDownload protocol
IHC protocol for GPR22 antibody 19951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols