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GPR22 Polyclonal antibody

GPR22 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19951-1-AP

Print datasheet

Synonyms

G-protein coupled receptor 22, G protein coupled receptor 22

验证数据展示

  • FC experiment of SH-SY5Y using 19951-1-AP

    FC experiment of SH-SY5Y using 19951-1-AP

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug GPR22 Polyclonal antibody (19951-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of mouse heart using 19951-1-AP

    WB analysis of mouse heart using 19951-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19951-1-AP (GPR22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 19951-1-AP

    IHC staining of human testis using 19951-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19951-1-AP (GPR22 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 19951-1-AP

    IHC staining of human testis using 19951-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19951-1-AP (GPR22 Antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19951-1-AP targets GPR22 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 22
Synonyms G-protein coupled receptor 22, G protein coupled receptor 22
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberNM_005295
Gene Symbol GPR22
Gene ID (NCBI) 2845
RRIDAB_10644129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99680
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR22 Belongs to the G-protein coupled receptor 1 family. GPR22 is an Orphan receptor. The antibody recognize the C-term of GPR22.

实验方案

Product Specific Protocols
WB protocol for GPR22 antibody 19951-1-APDownload protocol
IHC protocol for GPR22 antibody 19951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols