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验证数据展示

  • IHC staining of mouse small intestine using 30863-1-AP

    IHC staining of mouse small intestine using 30863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30863-1-AP (GPR52 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 30863-1-AP

    IF Staining of HepG2 using 30863-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPR52 antibody (30863-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IHC staining of mouse brain using 30863-1-AP

    IHC staining of mouse brain using 30863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30863-1-AP (GPR52 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR52 Polyclonal antibody

GPR52 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30863-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

G protein coupled receptor 52

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse small intestine tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30863-1-AP targets GPR52 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GPR52 fusion protein Ag31188 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 52
别名 G protein coupled receptor 52
计算分子量 361 aa, 41 kDa
GenBank蛋白编号BC067456
基因名称 GPR52
Gene ID (NCBI) 9293
RRIDAB_3669768
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2T5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR52, or G protein-coupled receptor 52, is an orphan G protein-coupled receptor (GPCR) that constitutively increases cellular cAMP levels and is coupled to the Gs protein, which activates adenylate cyclase. Agonism of GPR52 has been proposed as a therapeutic intervention for the psychotic and cognitive aspects of schizophrenia. Its role in modulating neurotransmission and the identification of ligands that can interact with it highlight its potential in therapeutic development.

实验方案

Product Specific Protocols
IHC protocol for GPR52 antibody 30863-1-APDownload protocol
IF protocol for GPR52 antibody 30863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols