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GPR65 Polyclonal antibody

GPR65 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20306-1-AP

Print datasheet

Synonyms

G protein coupled receptor 65, GPR65, hTDAG8, Psychosine receptor, TDAG8

验证数据展示

  • IHC staining of human tonsillitis using 20306-1-AP

    IHC staining of human tonsillitis using 20306-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 20306-1-AP

    IHC staining of human tonsillitis using 20306-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20306-1-AP targets GPR65 in IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen GPR65 fusion protein Ag14139 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 65
Synonyms G protein coupled receptor 65, GPR65, hTDAG8, Psychosine receptor, TDAG8
Calculated Molecular Weight 337 aa, 39 kDa
GenBank Accession NumberBC035633
Gene Symbol GPR65
Gene ID (NCBI) 8477
RRIDAB_2878668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYL9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR65 (G protein-coupled receptor 65) was defined originally as T-cell death-associated gene 8 (TDAG8), functions as a proton-sensing receptor, and is also sensitive to psychosine (PMID: 35343079). GPR65 promotes adaptation to an acidic environment to enhance cell survival and proliferation, thereby promoting tumor development (PMID: 36852075).

实验方案

Product Specific Protocols
IHC protocol for GPR65 antibody 20306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Neurosurg Rev

GPR65 contributes to constructing immunosuppressive microenvironment in glioma

Authors - Jikang Fan
View Article