验证数据展示
经过测试的应用
Positive IHC detected in | mouse brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue |
Positive IF/ICC detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17972-1-AP targets GPR75 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | IHC, IF/ICC, IF-P, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | GPR75 fusion protein Ag12053 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | G protein-coupled receptor 75 |
别名 | Probable G-protein coupled receptor 75 |
计算分子量 | 540 aa, 59 kDa |
GenBank蛋白编号 | BC067475 |
基因名称 | GPR75 |
Gene ID (NCBI) | 10936 |
RRID | AB_2878475 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O95800 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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IHC protocol for GPR75 antibody 17972-1-AP | Download protocol |
IF protocol for GPR75 antibody 17972-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |