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GPR75 Polyclonal antibody

GPR75 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 17972-1-AP

Print datasheet

Synonyms

Probable G-protein coupled receptor 75

验证数据展示

  • IHC staining of mouse brain using 17972-1-AP

    IHC staining of mouse brain using 17972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17972-1-AP (GPR75 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17972-1-AP

    IHC staining of mouse brain using 17972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17972-1-AP (GPR75 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 17972-1-AP

    IF Staining of rat brain using 17972-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GPR75 antibody (17972-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A549 using 17972-1-AP

    IF Staining of A549 using 17972-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using GPR75 antibody (17972-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17972-1-AP targets GPR75 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GPR75 fusion protein Ag12053 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 75
Synonyms Probable G-protein coupled receptor 75
Calculated Molecular Weight 540 aa, 59 kDa
GenBank Accession NumberBC067475
Gene Symbol GPR75
Gene ID (NCBI) 10936
RRIDAB_2878475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95800
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for GPR75 antibody 17972-1-APDownload protocol
IF protocol for GPR75 antibody 17972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols