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  • Featured Product
  • KD/KO Validated

GPR81 Polyclonal antibody, PBS Only

GPR81 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20146-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 20146-1-AP (same clone as 20146-1-PBS)

    WB analysis using 20146-1-AP (same clone as 20146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20146-1-AP (GPR81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

产品信息

20146-1-PBS targets GPR81 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen GPR81 fusion protein Ag13682 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 81
Synonyms
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC066881
Gene Symbol GPR81
Gene ID (NCBI) 27198
RRIDAB_2878647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXC0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPR81 (G protein-coupled receptor 81) is one of a large family of GPRs with low affinity for hydroxy-carboxylic acid structure ligands (PMID: 24657625). Lactate functions as a signaling molecule by serving as an agonist for the GPR81, involving both autocrine and paracrine mechanisms (PMID: 31836453). Moreover, A higher molecular mass band (∼60 kDa) is present in the brain and adipose tissue. This band is also strong in the transfected HeLa cells but weak in native HeLa cells and is therefore probably a form of GPR81, possibly a glycosylated form of the receptor (PMID: 23696276).