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GPRASP1-Specific Polyclonal antibody

GPRASP1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20020-1-AP

Print datasheet

Synonyms

GASP, GASP 1, GASP1, GPRASP1, GPRASP1-Specific, KIAA0443

验证数据展示

  • WB analysis of HeLa using 20020-1-AP

    WB analysis of HeLa using 20020-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20020-1-AP

    IHC staining of human brain using 20020-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 20020-1-AP

    IHC staining of human brain using 20020-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20020-1-AP targets GPRASP1-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor associated sorting protein 1
Synonyms GASP, GASP 1, GASP1, GPRASP1, GPRASP1-Specific, KIAA0443
Calculated Molecular Weight 157 kDa
Observed Molecular Weight 157 kDa
GenBank Accession NumberNM_014710
Gene Symbol GPRASP1
Gene ID (NCBI) 9737
RRIDAB_10694144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JY77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPRASP1, also named as GASP, GASP1 and KIAA0443, belongs to the GPRASP family. GPRASP1 may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It is targets receptors for degradation in lysosomes. The antibody is specific to GPRASP1.

实验方案

Product Specific Protocols
WB protocol for GPRASP1-Specific antibody 20020-1-APDownload protocol
IHC protocol for GPRASP1-Specific antibody 20020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

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