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GPRC5A,RAI3 Polyclonal antibody

GPRC5A,RAI3 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10309-1-AP

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Synonyms

GPRC5A, GPCR5A, RAI3, Phorbol ester induced gene 1, PEIG-1



经过测试的应用

Positive WB detected inA375 cells, MCF-7 cells, HeLa cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10309-1-AP targets GPRC5A,RAI3 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, goat
Immunogen GPRC5A,RAI3 fusion protein Ag0311 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor, family C, group 5, member A
Synonyms GPRC5A, GPCR5A, RAI3, Phorbol ester induced gene 1, PEIG-1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC003665
Gene Symbol GPRC5A
Gene ID (NCBI) 9052
RRIDAB_2877732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFJ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPRC5A, also named as GPCR5A, RAI3 and RAIG1, belongs to the G-protein coupled receptor 3 family. It is an orphan G-protein coupled receptor (GPCR) that has been associated with malignancy and may play a role in the proliferation of breast cancer cells. GPRC5A is a potential molecular target for treatment of breast cancers. It is suppressed in some human lung carcinoma cells. (PMID:15788639, 20563252, 19552806 )

实验方案

Product Specific Protocols
WB protocol for GPRC5A,RAI3 antibody 10309-1-APDownload protocol
IHC protocol for GPRC5A,RAI3 antibody 10309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
goatIHC

Cell Prolif

Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysis

Authors - Peng Lin
humanWB

Sci Total Environ

GPRC5A reduction contributes to pollutant benzo[a]pyrene injury via aggravating murine fibrosis, leading to poor prognosis of IIP patients.

Authors - Ziling Huang
  • KO Validated
humanWB

Oncol Lett

Clinical significance of reduced GPRC5A expression in surgically resected non-small cell lung cancer.

Authors - Er Jin
humanWB

Exp Lung Res

GPRC5a suppresses the proliferation of non-small cell lung cancer under wild type p53 background.

Authors - Jin Er

Mol Med Rep

Lung cancer suppressor gene GPRC5A mediates p53 activity in non‑small cell lung cancer cells in vitro.

Authors - Er Jin
humanWB

Cancers (Basel)

Identification of MYEOV-Associated Gene Network as a Potential Therapeutic Target in Pancreatic Cancer

Authors - Yu Chen