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验证数据展示

  • WB analysis using 21089-1-AP

    WB analysis using 21089-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21089-1-AP (GPRC5D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 21089-1-AP

    IHC staining of human kidney using 21089-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21089-1-AP (GPRC5D Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 21089-1-AP

    IHC staining of human kidney using 21089-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21089-1-AP (GPRC5D Antibody) at dilution of 1:100 (under 40x lens).

GPRC5D Polyclonal antibody

GPRC5D Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21089-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

G-protein coupled receptor family C group 5 member D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, THP-1 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21089-1-AP targets GPRC5D in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 GPRC5D fusion protein Ag15338 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor, family C, group 5, member D
别名 G-protein coupled receptor family C group 5 member D
计算分子量 345 aa, 39 kDa
观测分子量34-39 kDa
GenBank蛋白编号BC069341
基因名称 GPRC5D
Gene ID (NCBI) 55507
RRIDAB_2878810
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZD1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPRC5D antibody 21089-1-APDownload protocol
IHC protocol for GPRC5D antibody 21089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols