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GPRIN3-Specific Polyclonal antibody

GPRIN3-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20212-1-AP

Print datasheet

Synonyms

GPRIN3, KIAA2027, GRIN3, GPRIN family member 3, G protein-regulated inducer of neurite outgrowth 3

验证数据展示

  • WB analysis of human brain using 20212-1-AP

    WB analysis of human brain using 20212-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20212-1-AP (GPRIN3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 20212-1-AP

    IHC staining of human stomach using 20212-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20212-1-AP (GPRIN3-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue, mouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20212-1-AP targets GPRIN3-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GPRIN family member 3
Synonyms GPRIN3, KIAA2027, GRIN3, GPRIN family member 3, G protein-regulated inducer of neurite outgrowth 3
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 82 kDa
GenBank Accession NumberNM_198281
Gene Symbol GPRIN3
Gene ID (NCBI) 285513
RRIDAB_10666200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZVF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPRIN3, also named as KIAA2027, may be involved in neurite outgrowth. The antibody is specific to GPRIN3.

实验方案

Product Specific Protocols
WB protocol for GPRIN3-Specific antibody 20212-1-APDownload protocol
IHC protocol for GPRIN3-Specific antibody 20212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell Biol

UBE3D Regulates mRNA 3\'-End Processing and Maintains Adipogenic Potential in 3T3-L1 Cells

Authors - Daniel Heller-Trulli
View Article