GPT / ALT1 Polyclonal antibody
GPT / ALT1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue, mouse spleen tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 10 publications below |
产品信息
16897-1-AP targets GPT / ALT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | GPT / ALT1 fusion protein Ag10424 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | glutamic-pyruvate transaminase (alanine aminotransferase) |
| Synonyms | AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ ALT1, GPT1 |
| Calculated Molecular Weight | 496 aa, 55 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC018207 |
| Gene Symbol | GPT |
| Gene ID (NCBI) | 2875 |
| RRID | AB_2230815 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P24298 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPT / ALT1 antibody 16897-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations. | ||
Oncotarget Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft. | ||
J Proteome Res Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients. | ||
Mediators Inflamm Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury. | ||
Cancer Med Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1. |