验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse kidney tissue, HepG2 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16757-1-AP targets GPT2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, FC (Intra), ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GPT2 fusion protein Ag10273 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutamic pyruvate transaminase (alanine aminotransferase) 2 |
| 别名 | ALAT2, Alanine aminotransferase 2, Glutamic--pyruvic transaminase 2, Glutamic--alanine transaminase 2, EC:2.6.1.2 |
| 计算分子量 | 523 aa, 58 kDa |
| 观测分子量 | 58 kDa, 47 kDa |
| GenBank蛋白编号 | BC062555 |
| 基因名称 | GPT2 |
| Gene ID (NCBI) | 84706 |
| RRID | AB_2112098 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TD30 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPT2 (Alanine aminotransferase 2; ALT2) is a key enzyme in intermediatary metabolism, catalysing the reversible transfer of the amino group of glutamate to pyruvate, yielding alanine and ketoglutarate. GPT2 protein localizes to mitochondria, and is mainly expressed in the pancreas, brain, adrenal gland, skeletal muscle and heart. Serum GPT2 is a useful marker for liver damage. Recently mutation of GPT2 has been reported to be a cause of neural disorder (27601654). 16757-1-AP antibody recognizes two different isoforms of GPT2 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPT2 antibody 16757-1-AP | Download protocol |
| IHC protocol for GPT2 antibody 16757-1-AP | Download protocol |
| IF protocol for GPT2 antibody 16757-1-AP | Download protocol |
| IP protocol for GPT2 antibody 16757-1-AP | Download protocol |
| FC protocol for GPT2 antibody 16757-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations. | ||
Cell Res Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
| ||
Nat Commun Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner. | ||
Cancer Res Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma. | ||
Proc Natl Acad Sci U S A Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features. |
