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  • Featured Product
  • KD/KO Validated

GPT2 Polyclonal antibody

GPT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16757-1-AP

Print datasheet

Synonyms

ALAT2, Alanine aminotransferase 2, Glutamic--pyruvic transaminase 2, Glutamic--alanine transaminase 2, EC:2.6.1.2

验证数据展示

  • WB analysis using 16757-1-AP

    WB analysis using 16757-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16757-1-AP (GPT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 16757-1-AP

    WB analysis of HepG2 using 16757-1-AP

    WB result of GPT2 antibody (16757-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPT2 transfected HepG2 cells.

  • IHC staining of human skeletal muscle using 16757-1-AP

    IHC staining of human skeletal muscle using 16757-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16757-1-AP (GPT2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human skeletal muscle using 16757-1-AP

    IHC staining of human skeletal muscle using 16757-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16757-1-AP (GPT2 Antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of mouse kidney using 16757-1-AP

    IP experiment of mouse kidney using 16757-1-AP

    IP result of anti-GPT2 (IP:16757-1-AP, 4ug; Detection:16757-1-AP 1:500) with mouse kidney tissue lysate 7200ug.

  • FC experiment of HepG2 using 16757-1-AP

    FC experiment of HepG2 using 16757-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human GPT2 (16757-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 16757-1-AP

    IF Staining of HepG2 using 16757-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT2 antibody (16757-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse liver tissue, mouse kidney tissue, HepG2 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16757-1-AP targets GPT2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, FC (Intra), ELISA
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen GPT2 fusion protein Ag10273 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamic pyruvate transaminase (alanine aminotransferase) 2
Synonyms ALAT2, Alanine aminotransferase 2, Glutamic--pyruvic transaminase 2, Glutamic--alanine transaminase 2, EC:2.6.1.2
Calculated Molecular Weight 523 aa, 58 kDa
Observed Molecular Weight 58 kDa, 47 kDa
GenBank Accession NumberBC062555
Gene Symbol GPT2
Gene ID (NCBI) 84706
RRIDAB_2112098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TD30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPT2 (Alanine aminotransferase 2; ALT2) is a key enzyme in intermediatary metabolism, catalysing the reversible transfer of the amino group of glutamate to pyruvate, yielding alanine and ketoglutarate. GPT2 protein localizes to mitochondria, and is mainly expressed in the pancreas, brain, adrenal gland, skeletal muscle and heart. Serum GPT2 is a useful marker for liver damage. Recently mutation of GPT2 has been reported to be a cause of neural disorder (27601654). 16757-1-AP antibody recognizes two different isoforms of GPT2 protein.

实验方案

Product Specific Protocols
WB protocol for GPT2 antibody 16757-1-APDownload protocol
IHC protocol for GPT2 antibody 16757-1-APDownload protocol
IF protocol for GPT2 antibody 16757-1-APDownload protocol
IP protocol for GPT2 antibody 16757-1-APDownload protocol
FC protocol for GPT2 antibody 16757-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cancer Cell

Inosine induces stemness features in CAR-T cells and enhances potency

Authors - Dorota D Klysz
View Article
WB

Cell Metab

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Authors - Qiuying Chen
View Article
mouseWB

Cell Res

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Authors - Wei-Feng Cai
  • KD Validated
View Article
humanWB

Nat Commun

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Authors - Yun-Qian Wang
View Article
humanWB

Cancer Res

Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.

Authors - Bo Zhang
View Article
MOUSEWB,IF

Proc Natl Acad Sci U S A

Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.

Authors - Qing Ouyang
View Article