验证数据展示

WB analysis using 30388-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30388-1-AP (GPX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 30388-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30388-1-AP (GPX4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
FC experiment of HeLa using 30388-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GPX4 (30388-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IF Staining of rat testis using 30388-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat testis tissue using GPX4 antibody (30388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat testis using 30388-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat testis tissue using GPX4 antibody (30388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB analysis of HEK-293 using 30388-1-AP
WB result of GPX4 antibody (30388-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 transfected HEK-293 cells.
IP experiment of rat testis using 30388-1-AP
IP result of anti-GPX4 (IP:30388-1-AP, 4ug; Detection:30388-1-AP 1:3000) with rat testis tissue lysate 1880 ug.

Featured Product
KD/KO验证

GPX4 Polyclonal antibody

GPX4 Polyclonal Antibody for WB, IF-P, FC (Intra), IP, ELISA

Cat No. 30388-1-AP

产品说明书

引用文献(77)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IF-P, FC (Intra), IP, ELISA and More (2)

别名

GSHPx4, GSHPx 4, GPx-4, GPx 4, EC:1.11.1.9

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格:

-/ -

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (77)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HCT 116 cells, HL-60 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IP detected in	rat testis tissue
Positive IF-P detected in	rat testis tissue
Positive FC (Intra) detected in	HeLa cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 70 publications below
IHC	See 13 publications below
IF	See 11 publications below
IP	See 1 publications below
FC	See 1 publications below

产品信息

30388-1-AP targets GPX4 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig
免疫原	GPX4 fusion protein Ag30650 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	glutathione peroxidase 4 (phospholipid hydroperoxidase)
别名	GSHPx4, GSHPx 4, GPx-4, GPx 4, EC:1.11.1.9
观测分子量	18-23 kDa
GenBank蛋白编号	BC021567
基因名称	GPX4
Gene ID (NCBI)	2879
RRID	AB_3086304
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P36969
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

实验方案

Product Specific Protocols
WB protocol for GPX4 antibody 30388-1-AP	Download protocol
IF protocol for GPX4 antibody 30388-1-AP	Download protocol
IP protocol for GPX4 antibody 30388-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse,rat	WB,IHC,IF	Bioact Mater A two-pronged approach to inhibit ferroptosis of MSCs caused by the iron overload in postmenopausal osteoporosis and promote osseointegration of titanium implant Authors - Yulu Yang View Article
human,mouse	WB,IF	Sci Adv In situ implantable DNA hydrogel for diagnosis and therapy of postoperative rehemorrhage following intracerebral hemorrhage surgery Authors - Wenyan Yu View Article
mouse	WB	J Hazard Mater Investigating the potential risk of cadmium exposure on seizure severity and anxiety-like behaviors through the ferroptosis pathway in epileptic mice: An integrated multi-omics approach Authors - Yuanjin Chang View Article
human	WB,IHC	Adv Healthc Mater Fe3 O4 -Incorporated Metal-Organic Framework for Chemo/Ferroptosis Synergistic Anti-Tumor via the Enhanced Chemodynamic Therapy Authors - Yuliang Xu View Article
rat	WB	Biomed Pharmacother AP39 inhibits ferroptosis by inhibiting mitochondrial autophagy through the PINK1/parkin pathway to improve myocardial fibrosis with myocardial infarction Authors - Ting Yang View Article
mouse	WB,IF	Cells Unveiling Smyd-2's Role in Cytoplasmic Nrf-2 Sequestration and Ferroptosis Induction in Hippocampal Neurons After Cerebral Ischemia/Reperfusion Authors - Daohang Liu View Article

验证数据展示

WB analysis using 30388-1-AP

WB analysis using 30388-1-AP

FC experiment of HeLa using 30388-1-AP

IF Staining of rat testis using 30388-1-AP

IF Staining of rat testis using 30388-1-AP

WB analysis of HEK-293 using 30388-1-AP

IP experiment of rat testis using 30388-1-AP

GPX4 Polyclonal antibody

GPX4 Polyclonal Antibody for WB, IF-P, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

A two-pronged approach to inhibit ferroptosis of MSCs caused by the iron overload in postmenopausal osteoporosis and promote osseointegration of titanium implant

In situ implantable DNA hydrogel for diagnosis and therapy of postoperative rehemorrhage following intracerebral hemorrhage surgery

Investigating the potential risk of cadmium exposure on seizure severity and anxiety-like behaviors through the ferroptosis pathway in epileptic mice: An integrated multi-omics approach

Fe3 O4 -Incorporated Metal-Organic Framework for Chemo/Ferroptosis Synergistic Anti-Tumor via the Enhanced Chemodynamic Therapy

AP39 inhibits ferroptosis by inhibiting mitochondrial autophagy through the PINK1/parkin pathway to improve myocardial fibrosis with myocardial infarction

Unveiling Smyd-2's Role in Cytoplasmic Nrf-2 Sequestration and Ferroptosis Induction in Hippocampal Neurons After Cerebral Ischemia/Reperfusion