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GPX7 Polyclonal antibody
GPX7 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, human placenta tissue, human brain tissue, Transfected HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GPX7 Ready-To-Use IHC Kit. GPX7 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13501-1-AP targets GPX7 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, chicken |
| Immunogen | GPX7 fusion protein Ag4328 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | glutathione peroxidase 7 |
| Synonyms | CL683, glutathione peroxidase 7, GPx 7, GPX6, GPX7, GSHPx 7 |
| Calculated Molecular Weight | 187 aa, 21 kDa |
| Observed Molecular Weight | 18-21 kDa |
| GenBank Accession Number | BC032788 |
| Gene Symbol | GPX7 |
| Gene ID (NCBI) | 2882 |
| RRID | AB_2112560 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96SL4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPX7(Glutathione peroxidase 7) is also named as GPX6,CL683,GSHPx-7 and belongs to the glutathione peroxidase family.It shares approximately 45% amino acid sequence with other GPX family members.Because GPX7 expression protected cells from acidic bile acidinduced DNA damage, it may protect oesophageal epithelial cells from acidic bile acid-induced apoptosis(PMID:22157330). This antibody can ve detected as 18-21kDa due to signal peptide splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPX7 antibody 13501-1-AP | Download protocol |
| IHC protocol for GPX7 antibody 13501-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gut Glutathione peroxidase 7 has potential tumour suppressor functions that are silenced by location-specific methylation in oesophageal adenocarcinoma. | ||
EMBO Mol Med Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human. | ||
Oncogene Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling. | ||
Carcinogenesis Loss of glutathione peroxidase 7 promotes TNF-α-induced NF-κB activation in Barrett's carcinogenesi.
| ||
Free Radic Biol Med GPx8 peroxidase prevents leakage of H2O2 from the endoplasmic reticulum. |
