GRAF Recombinant antibody
GRAF Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240086A11
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, A549 cells, Jurkat cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83206-1-RR targets GRAF in WB, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | GRAF fusion protein Ag12008 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | Rho GTPase activating protein 26 |
| Synonyms | ARHGAP26, 240086A11, Oligophrenin-1-like protein, Oligophrenin 1 like protein, KIAA0621 |
| Calculated Molecular Weight | 759 aa, 86 kDa |
| Observed Molecular Weight | 92-100 kDa |
| GenBank Accession Number | BC068555 |
| Gene Symbol | ARHGAP26 |
| Gene ID (NCBI) | 23092 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UNA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTPase Regulator Associated with Focal Adhesion Kinase (GRAF), also known as Rho GTPase activating protein 26 (ARHGAP26) , is a GTPase-activating protein and inhibits the activity of Rho GTPases by promoting the hydrolytic ability of Rho GTPases. GRAF enhances the hydrolysis of GTPases and converts GTPases from an active form to an inactive form, thereby inhibiting signaling transduction. Deletion and mutation of ARHGAP26 can lead to promyelocytic leukemia, suggesting tumor suppressive activity of ARHGAP26. ARHGAP26 was downregulated in glioblastoma and associated with cell proliferation and migration (PMID: 10908648, PMID: 17611651, PMID: 31004081).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRAF antibody 83206-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |