验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293T cells, HeLa cells, SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25487-1-AP targets GRAMD1A in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | GRAMD1A fusion protein Ag22221 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GRAM domain containing 1A |
| 别名 | GRAM domain containing 1A, GRAMD1A, KIAA1533 |
| 计算分子量 | 724 aa, 81 kDa |
| 观测分子量 | 80-90 kDa |
| GenBank蛋白编号 | BC014077 |
| 基因名称 | GRAMD1A |
| Gene ID (NCBI) | 57655 |
| RRID | AB_2935462 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96CP6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein Aster-A (GRAMD1A) is a cholesterol transporter, which contains unique domains for binding cholesterol and the plasma membrane (PM), thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the endoplasmic reticulum (ER). It was reported that plays a role in autophagy regulation and is required for the biogenesis of the autophagosome (PMID:31222192). It can be detected as 80 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRAMD1A antibody 25487-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |