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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 23591-1-AP (same clone as 23591-1-PBS)

    WB analysis using 23591-1-AP (same clone as 23591-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23591-1-AP (GRB10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23591-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 23591-1-AP (same clone as 23591-1-PBS)

    IF Staining of HepG2 using 23591-1-AP (same clone as 23591-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRB10 antibody (23591-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23591-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 23591-1-AP (same clone as 23591-1-PBS)

    IHC staining of human liver using 23591-1-AP (same clone as 23591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23591-1-AP (GRB10 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23591-1-PBS in a different storage buffer formulation.

GRB10 Polyclonal antibody, PBS Only

GRB10 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23591-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

23591-1-PBS targets GRB10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GRB10 fusion protein Ag20355 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth factor receptor-bound protein 10
别名
计算分子量536aa,61 kDa; 594aa,67 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC024285
基因名称 GRB10
Gene ID (NCBI) 2887
RRIDAB_2879301
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13322
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRB10 is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation.