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GRB14 Polyclonal antibody

GRB14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15298-1-AP

Print datasheet

Synonyms

GRB14, GRB14 adapter protein

验证数据展示

  • IHC staining of human ovary tumor using 15298-1-AP

    IHC staining of human ovary tumor using 15298-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15298-1-AP (GRB14 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary tumor using 15298-1-AP

    IHC staining of human ovary tumor using 15298-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15298-1-AP (GRB14 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of DU 145 using 15298-1-AP

    WB analysis of DU 145 using 15298-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 15298-1-AP (GRB14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inDU 145 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15298-1-AP targets GRB14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GRB14 fusion protein Ag7554 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name growth factor receptor-bound protein 14
Synonyms GRB14, GRB14 adapter protein
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC053559
Gene Symbol GRB14
Gene ID (NCBI) 2888
RRIDAB_2112892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14449
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRB14 belongs to the GRB7/10/14 family. It is an adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. GRB14 is a target for a PDGF regulated serine kinase, an interaction that does not require PDGFR-GRB14 association. GRB14 is about 58-65kd in WB test.

实验方案

Product Specific Protocols
WB protocol for GRB14 antibody 15298-1-APDownload protocol
IHC protocol for GRB14 antibody 15298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols