验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy GRB7 Ready-To-Use IHC Kit. GRB7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10045-1-Ig targets GRB7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | GRB7 fusion protein Ag0023 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | growth factor receptor-bound protein 7 |
| 别名 | Growth factor receptor-bound protein 7, GRB7 adapter protein, Epidermal growth factor receptor GRB-7, B47 |
| 计算分子量 | 532 aa, 60 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC006535 |
| 基因名称 | GRB7 |
| Gene ID (NCBI) | 2886 |
| RRID | AB_2232601 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14451 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Grb7 belongs to a family of adapter proteins. This family, including Grb10, Grb7 and Grb 14, contains a praline-rich, a RA, a PH, a BPS and SH2 domain. Grb7 associate with a number of receptors including EGFR and FAK. Functions of Grb7 include mobility because it associates receptors such FAK, and direct evidence from overexpression of this proteins.This antibody recognize the N-terminal of GRB7.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRB7 antibody 10045-1-Ig | Download protocol |
| IHC protocol for GRB7 antibody 10045-1-Ig | Download protocol |
| IF protocol for GRB7 antibody 10045-1-Ig | Download protocol |
| IP protocol for GRB7 antibody 10045-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol Astragalus propinquus schischkin and Salvia miltiorrhiza bunge promote angiogenesis to treat myocardial ischemia via Ang-1/Tie-2/FAK pathway | ||
Bioengineered Knockdown of growth factor receptor bound protein 7 suppresses angiogenesis by inhibiting the secretion of vascular endothelial growth factor A in ovarian cancer cells.
| ||
J Transl Med TCF12-regulated GRB7 facilitates the HER2+ breast cancer progression by activating Notch1 signaling pathway | ||
Pharmaceuticals (Basel) GRB7 Plays a Vital Role in Promoting the Progression and Mediating Immune Evasion of Ovarian Cancer
| ||
Front Microbiol GRB7-mediated enhancement of cell malignant characteristics induced by Helicobacter pylori infection | ||
Mol Carcinog TRIM36 Inhibits the Development of AOM/DSS-Induced Colitis-Associated Colorectal Cancer by Promoting the Ubiquitination and Degradation of GRB7 |
