验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue, human liver tissue, L02 cells, MCF-7 cells |
Positive IHC detected in | human liver tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
51013-2-AP targets GRHPR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | GRHPR fusion protein Ag0484 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | glyoxylate reductase/hydroxypyruvate reductase |
别名 | GLXR, GLYD, GRHPR, PH2 |
计算分子量 | 328 aa, 36 kDa |
观测分子量 | 36 kDa |
GenBank蛋白编号 | BC000605 |
基因名称 | GRHPR |
Gene ID (NCBI) | 9380 |
RRID | AB_2113424 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UBQ7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRHPR(Glyoxylate reductase/hydroxypyruvate reductase) is also named as GLXR and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The GRHPR gene encodes a predicted 328-amino acid protein with a calculated molecular mass of 35.5 kD. It has glyoxylate reductase (GR), hydroxypyruvate reductase (HPR) and D-glycerate dehydrogenase (DGDH) activities(PMID:16597637). Defects in GRHPR are the cause of hyperoxaluria primary type 2 (HP2)(PMID: 10484776). It can exsit as a homodimer(PMID:16756993).
实验方案
Product Specific Protocols | |
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WB protocol for GRHPR antibody 51013-2-AP | Download protocol |
IHC protocol for GRHPR antibody 51013-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |