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GRHPR Polyclonal antibody

GRHPR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51013-2-AP

Print datasheet

Synonyms

GLXR, GLYD, GRHPR, PH2

验证数据展示

  • WB analysis of mouse liver using 51013-2-AP

    WB analysis of mouse liver using 51013-2-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 51013-2-AP

    IHC staining of human liver using 51013-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51013-2-AP (GRHPR Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with GRHPR antibody included
  • IHC staining of human liver using 51013-2-AP

    IHC staining of human liver using 51013-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51013-2-AP (GRHPR Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with GRHPR antibody included

经过测试的应用

Positive WB detected inmouse liver tissue, human liver tissue, L02 cells, MCF-7 cells
Positive IHC detected inhuman liver tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GRHPR Ready-To-Use IHC Kit. GRHPR primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51013-2-AP targets GRHPR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GRHPR fusion protein Ag0484 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glyoxylate reductase/hydroxypyruvate reductase
Synonyms GLXR, GLYD, GRHPR, PH2
Calculated Molecular Weight 328 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000605
Gene Symbol GRHPR
Gene ID (NCBI) 9380
RRIDAB_2113424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBQ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRHPR(Glyoxylate reductase/hydroxypyruvate reductase) is also named as GLXR and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The GRHPR gene encodes a predicted 328-amino acid protein with a calculated molecular mass of 35.5 kD. It has glyoxylate reductase (GR), hydroxypyruvate reductase (HPR) and D-glycerate dehydrogenase (DGDH) activities(PMID:16597637). Defects in GRHPR are the cause of hyperoxaluria primary type 2 (HP2)(PMID: 10484776). It can exsit as a homodimer(PMID:16756993).

实验方案

Product Specific Protocols
WB protocol for GRHPR antibody 51013-2-APDownload protocol
IHC protocol for GRHPR antibody 51013-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols