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Glutamate receptor 3/GluA3 Polyclonal antibody

Glutamate receptor 3/GluA3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 29588-1-AP

Print datasheet

Synonyms

GRIA3, GLURC, GLUR3, GLUR K3, GLUR C

验证数据展示

  • WB analysis using 29588-1-AP

    WB analysis using 29588-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29588-1-AP (Glutamate receptor 3/GluA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 29588-1-AP

    IP experiment of mouse brain using 29588-1-AP

    IP result of anti-Glutamate receptor 3/GluA3 (IP:29588-1-AP, 4ug; Detection:29588-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

29588-1-AP targets Glutamate receptor 3/GluA3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Glutamate receptor 3/GluA3 fusion protein Ag30898 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamate receptor, ionotrophic, AMPA 3
Synonyms GRIA3, GLURC, GLUR3, GLUR K3, GLUR C
Calculated Molecular Weight 101 kDa
Observed Molecular Weight101 kDa
GenBank Accession NumberNM_007325
Gene Symbol GRIA3
Gene ID (NCBI) 2892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42263
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA3 (Glutamate ionotropic receptor AMPA type subunit 3), also known as Glutamate receptor R3 or A3 (GluR3 or GluA3), is associated with X-linked intellectual disability (ID), dysmorphic features, and non-syndromic epilepsy(PMID: 32977175). GRIA3 also plays a role as a mediator of tumor progression in pancreatic cancer downstream CUX1(PMID: 20689760).

实验方案

Product Specific Protocols
WB protocol for Glutamate receptor 3/GluA3 antibody 29588-1-APDownload protocol
IP protocol for Glutamate receptor 3/GluA3 antibody 29588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

iScience

The decreased astrocyte-microglia interaction reflects the early characteristics of Alzheimer's disease

Authors - Kefu Liu
View Article