验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IF-P detected in | rat brain tissue, mouse brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IHC | See 1 publications below |
产品信息
29588-1-AP targets Glutamate receptor 3/GluA3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF-P, IP, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | Glutamate receptor 3/GluA3 fusion protein Ag30898 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | glutamate receptor, ionotrophic, AMPA 3 |
别名 | GRIA3, GLURC, GLUR3, GLUR K3, GLUR C |
计算分子量 | 101 kDa |
观测分子量 | 101 kDa |
GenBank蛋白编号 | NM_007325 |
基因名称 | GRIA3 |
Gene ID (NCBI) | 2892 |
RRID | AB_3086141 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P42263 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA3 (Glutamate ionotropic receptor AMPA type subunit 3), also known as Glutamate receptor R3 or A3 (GluR3 or GluA3), is associated with X-linked intellectual disability (ID), dysmorphic features, and non-syndromic epilepsy(PMID: 32977175). GRIA3 also plays a role as a mediator of tumor progression in pancreatic cancer downstream CUX1(PMID: 20689760).
实验方案
Product Specific Protocols | |
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WB protocol for Glutamate receptor 3/GluA3 antibody 29588-1-AP | Download protocol |
IF protocol for Glutamate receptor 3/GluA3 antibody 29588-1-AP | Download protocol |
IP protocol for Glutamate receptor 3/GluA3 antibody 29588-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Discov Postsynaptic lncRNA Sera/Pkm2 pathway orchestrates the transition from social competition to rank by remodeling the neural ensemble in mPFC | ||
Brain Res Low-dose diazepam improves cognitive function in APP/PS1 mouse models: Involvement of AMPA receptors | ||
iScience The decreased astrocyte-microglia interaction reflects the early characteristics of Alzheimer's disease |