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GRID1 Polyclonal antibody

GRID1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13040-1-AP

Print datasheet

Synonyms

KIAA1220, Glutamate receptor ionotropic, delta-1, GluR delta-1 subunit, GluR delta 1 subunit, GluD1

验证数据展示

  • WB analysis of human brain using 13040-1-AP

    WB analysis of human brain using 13040-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13040-1-AP (GRID1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 13040-1-AP

    IHC staining of human gliomas using 13040-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 13040-1-AP

    IHC staining of human gliomas using 13040-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 13040-1-AP

    IP experiment of mouse brain using 13040-1-AP

    IP result of anti-GRID1 (IP:13040-1-AP, 4ug; Detection:13040-1-AP 1:300) with mouse brain tissue lysate 5600ug.

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13040-1-AP targets GRID1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen GRID1 fusion protein Ag3694 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamate receptor, ionotropic, delta 1
Synonyms KIAA1220, Glutamate receptor ionotropic, delta-1, GluR delta-1 subunit, GluR delta 1 subunit, GluD1
Calculated Molecular Weight 1009 aa, 112 kDa
Observed Molecular Weight 100-112 kDa
GenBank Accession NumberBC039263
Gene Symbol GRID1
Gene ID (NCBI) 2894
RRIDAB_10638439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULK0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRID1 (Glutamate Ionotropic Receptor Delta Type Subunit 1) is a protein-coding gene that belongs to the family of ionotropic glutamate receptors. These receptors are crucial for rapid excitatory synaptic transmission and synaptic plasticity in the central nervous system. GRID1 is primarily expressed in various brain regions, including the cortex, hippocampus, amygdala, striatum, thalamus, nucleus accumbens, lateral hooked nucleus, and dorsal raphe nucleus. In the hippocampus, GRID1 is specifically expressed in the granule cells of the dentate gyrus.

实验方案

Product Specific Protocols
WB protocol for GRID1 antibody 13040-1-APDownload protocol
IHC protocol for GRID1 antibody 13040-1-APDownload protocol
IP protocol for GRID1 antibody 13040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Anesthesiology

Single-nucleus Atlas of Sevoflurane-induced Hippocampal Cell Type- and Sex-specific Effects during Development in Mice

Authors - Shao-Yong Song
View Article