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GRIK1 Polyclonal antibody

GRIK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25779-1-AP

Print datasheet

Synonyms

EAA3, EEA3, GLR5, GluK1, GluR 5, GLUR5, Glutamate receptor 5, GRIK1

验证数据展示

  • WB analysis using 25779-1-AP

    WB analysis using 25779-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25779-1-AP (GRIK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 25779-1-AP

    IHC staining of human cerebellum using 25779-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25779-1-AP (GRIK1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human cerebellum using 25779-1-AP

    IHC staining of human cerebellum using 25779-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25779-1-AP (GRIK1 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25779-1-AP is GRIK1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GRIK1 fusion protein Ag22664 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamate receptor, ionotropic, kainate 1
Synonyms EAA3, EEA3, GLR5, GluK1, GluR 5, GLUR5, Glutamate receptor 5, GRIK1
Calculated Molecular Weight 918 aa, 104 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC156975
Gene Symbol GRIK1
Gene ID (NCBI) 2897
RRIDAB_2880236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP39086
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRIK1 antibody 25779-1-APDownload protocol
IHC protocol for GRIK1 antibody 25779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Exp Ther Med

Glutamate receptor ionotropic, kainate 1 serves as a novel tumor suppressor of colorectal carcinoma and predicts clinical prognosis.

Authors - Zhong Ren
View Article
humanWB,IHC

Oncol Res

GRIK1 promotes glioblastoma malignancy and is a novel prognostic factor of poor prognosis

Authors - Guoqiang Hou
View Article