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Species Reactivity
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验证数据展示

  • WB analysis using 25779-1-AP

    WB analysis using 25779-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25779-1-AP (GRIK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 25779-1-AP

    IHC staining of human cerebellum using 25779-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25779-1-AP (GRIK1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human cerebellum using 25779-1-AP

    IHC staining of human cerebellum using 25779-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25779-1-AP (GRIK1 antibody) at dilution of 1:200 (under 40x lens).

GRIK1 Polyclonal antibody

GRIK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25779-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

EAA3, EEA3, GLR5, GluK1, GluR 5, GLUR5, Glutamate receptor 5, GRIK1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25779-1-AP targets GRIK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 GRIK1 fusion protein Ag22664 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutamate receptor, ionotropic, kainate 1
别名 EAA3, EEA3, GLR5, GluK1, GluR 5, GLUR5, Glutamate receptor 5, GRIK1
计算分子量 918 aa, 104 kDa
观测分子量 95-100 kDa
GenBank蛋白编号BC156975
基因名称 GRIK1
Gene ID (NCBI) 2897
RRIDAB_2880236
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP39086
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRIK1 antibody 25779-1-APDownload protocol
IHC protocol for GRIK1 antibody 25779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Exp Ther Med

Glutamate receptor ionotropic, kainate 1 serves as a novel tumor suppressor of colorectal carcinoma and predicts clinical prognosis.

Authors - Zhong Ren
View Article
humanWB,IHC

Oncol Res

GRIK1 promotes glioblastoma malignancy and is a novel prognostic factor of poor prognosis

Authors - Guoqiang Hou
View Article