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验证数据展示

  • WB analysis using 28550-1-AP

    WB analysis using 28550-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28550-1-AP (GRIK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28550-1-AP

    IHC staining of mouse brain using 28550-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28550-1-AP (GRIK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GRIK5 Polyclonal antibody

GRIK5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28550-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

EAA2, Glutamate receptor KA 2, GRIK2, GRIK5, KA2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28550-1-AP targets GRIK5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 GRIK5 fusion protein Ag29363 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutamate receptor, ionotropic, kainate 5
别名 EAA2, Glutamate receptor KA 2, GRIK2, GRIK5, KA2
计算分子量 109 kDa
观测分子量~110 kDa
GenBank蛋白编号NM_001301030
基因名称 GRIK5
Gene ID (NCBI) 2901
RRIDAB_3086064
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16478
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRIK5 antibody 28550-1-APDownload protocol
IHC protocol for GRIK5 antibody 28550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols