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验证数据展示

  • WB analysis of LNCaP using 67414-1-Ig (same clone as 67414-1-PBS)

    WB analysis of LNCaP using 67414-1-Ig (same clone as 67414-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67414-1-Ig (same clone as 67414-1-PBS)

    WB analysis of HepG2 using 67414-1-Ig (same clone as 67414-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFA13 antibody included
  • IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFA13 antibody included
  • Cytometric bead array standard curve of MP50735-1

    Cytometric bead array standard curve of MP50735-1

    Cytometric bead array standard curve of MP50735-1, GRIM19 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67414-2-PBS. Detection antibody: 67414-1-PBS. Standard:Ag20855. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50735-3

    Cytometric bead array standard curve of MP50735-3

    Cytometric bead array standard curve of MP50735-3, GRIM19 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67414-1-PBS. Detection antibody: 67414-4-PBS. Standard:Ag20855. Range: 0.098-100 ng/mL.

GRIM19 Monoclonal antibody, PBS Only (Capture/Detector)

GRIM19 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 67414-1-PBS

产品说明书

CloneNo. 3A10C5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

NDUFA13, CI B16.6, CGI-39, CGI 39, Cell death regulatory protein GRIM-19

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NDUFA13 Ready-To-Use IHC Kit. NDUFA13 primary antibody included.

产品信息

67414-1-PBS targets GRIM19 as part of a matched antibody pair:

MP50735-1: 67414-2-PBS capture and 67414-1-PBS detection (validated in Cytometric bead array)

MP50735-3: 67414-1-PBS capture and 67414-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 GRIM19 fusion protein Ag20855 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
别名 NDUFA13, CI B16.6, CGI-39, CGI 39, Cell death regulatory protein GRIM-19
计算分子量16.5 kDa
观测分子量 16-18 kDa
GenBank蛋白编号BC009189
基因名称 GRIM19
Gene ID (NCBI) 51079
RRIDAB_2882654
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9P0J0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRIM19(Gene associated with retinoic and interferon-induced mortality 19 protein) is also named as NDUFA13 ,CI-B16.6 and belongs to the complex I NDUFA13 subunit family.t GRIM-19 may distribute in the mitochondria and/or nucleus depending on the cell type.Alternatively, its cellular localization may be regulated by post-translational modifications such as phosphorylation and acetylation. Lastly, GRIM-19 may exist in different isoforms that may be distributed in distinct cellular locations(PMID:12628925).