NMDAR1/GRIN1 Polyclonal antibody

NMDAR1/GRIN1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 27676-1-AP

Print datasheet

Synonyms

GRIN1, NMDAR1, NMD-R1, NMDAR 1, Glutamate receptor ionotropic, NMDA 1



经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27676-1-AP targets NMDAR1/GRIN1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen NMDAR1/GRIN1 fusion protein Ag26364 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 1
Synonyms GRIN1, NMDAR1, NMD-R1, NMDAR 1, Glutamate receptor ionotropic, NMDA 1
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberNM_000832
Gene Symbol NMDAR1
Gene ID (NCBI) 2902
RRIDAB_2880944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NMDAR1/GRIN1 antibody 27676-1-APDownload protocol
IHC protocol for NMDAR1/GRIN1 antibody 27676-1-APDownload protocol
IF protocol for NMDAR1/GRIN1 antibody 27676-1-APDownload protocol
IP protocol for NMDAR1/GRIN1 antibody 27676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Adv

RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission

Authors - Pengcheng Ma
ratWB

Aging (Albany NY)

EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment.

Authors - Zhao-Hui Yao
mouseWB

Exp Neurol

DPP-4 inhibition by linagliptin ameliorates age-related mild cognitive impairment by regulating microglia polarization in mice

Authors - Fen Zhuge
ratWB,IP

Heliyon

Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1

Authors - Chuan Jiang
mouseWB

Cell Discov

Postsynaptic lncRNA Sera/Pkm2 pathway orchestrates the transition from social competition to rank by remodeling the neural ensemble in mPFC

Authors - Ling-Shuang Zhu
humanWB,IHC

Life Sci

Identification of a robust scoring system based on metabolic genes followed by in-depth validation of ATP1A3 in glioma

Authors - Qiankun Ji