GRIPAP1 Polyclonal antibody

GRIPAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15806-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

KIAA1167, GRIP1-associated protein 1, GRIP1 associated protein 1, GRASP-1 C-terminal chain, GRASP-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15806-1-AP targets GRIPAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 GRIPAP1 fusion protein Ag8540 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GRIP1 associated protein 1
别名 KIAA1167, GRIP1-associated protein 1, GRIP1 associated protein 1, GRASP-1 C-terminal chain, GRASP-1
计算分子量 841 aa, 96 kDa
观测分子量 97 kDa
GenBank蛋白编号BC001522
基因名称 GRIPAP1
Gene ID (NCBI) 56850
RRIDAB_2112787
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4V328
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRIP-associated protein-1 (GRASP-1), also named as GRIPAP1, is a neuron-specific effector of the small GTPase Rab4 and key component of AMPA receptor recycling machinery in dendrites. GRASP-1 is essential for maintenance of spine morphology and important for LTP. GRASP-1 connects Rab4 and Rab11 recycling endosomal domains through the interaction with target (t)-SNARE syntaxin. The MW of this protein is 97 kDa, and Catalog#15806-1-AP specially recognises the 97 kDa protein.

实验方案

Product Specific Protocols
WB protocol for GRIPAP1 antibody 15806-1-APDownload protocol
IHC protocol for GRIPAP1 antibody 15806-1-APDownload protocol
IF protocol for GRIPAP1 antibody 15806-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression.

Authors - Hani Goodarzi
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