GRK1 Polyclonal antibody
GRK1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF-P, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, mouse eye tissue |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24606-1-AP targets GRK1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF-P, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse, zebrafish |
| Immunogen | GRK1 fusion protein Ag18063 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | G protein-coupled receptor kinase 1 |
| Synonyms | RHOK, Rhodopsin kinase GRK1, Rhodopsin kinase, GPRK1, G protein-coupled receptor kinase 1 |
| Calculated Molecular Weight | 563 aa, 64 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC140945 |
| Gene Symbol | GRK1 |
| Gene ID (NCBI) | 6011 |
| RRID | AB_2879635 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15835 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRK1(G protein-coupled receptor kinase 1) is also named as RHOK, RK, GPRK1 and belongs to the AGC Ser/Thr protein kinase family. It is involved in quenching of light-induced signal transduction in phosphoreceptor. GRK1 is a photoreceptor-specific enzyme that mediates adaptation of photoreceptors to light and protects these cells against light-induced injury.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRK1 antibody 24606-1-AP | Download protocol |
| IHC protocol for GRK1 antibody 24606-1-AP | Download protocol |
| IF protocol for GRK1 antibody 24606-1-AP | Download protocol |
| IP protocol for GRK1 antibody 24606-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Zool Res Characterization of two novel knock-in mouse models of syndromic retinal ciliopathy carrying hypomorphic Sdccag8 mutations. | ||
Biochim Biophys Acta Mol Basis Dis Knockout of DLIC1 leads to retinal cone degeneration via disturbing Rab8 transport in zebrafish |
