• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human lymphoma using 13990-1-AP (same clone as 13990-1-PBS)

    IHC staining of human lymphoma using 13990-1-AP (same clone as 13990-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

  • WB analysis using 13990-1-AP (same clone as 13990-1-PBS)

    WB analysis using 13990-1-AP (same clone as 13990-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13990-1-AP (GRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

  • IP experiment of HL-60 using 13990-1-AP (same clone as 13990-1-PBS)

    IP experiment of HL-60 using 13990-1-AP (same clone as 13990-1-PBS)

    IP result of anti-GRK2 (IP:13990-1-AP, 4ug; Detection:13990-1-AP 1:1000) with HL-60 cells lysate 3040ug. This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 13990-1-AP (same clone as 13990-1-PBS)

    FC experiment of HeLa using 13990-1-AP (same clone as 13990-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug GRK2 Polyclonal antibody (13990-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 13990-1-AP (same clone as 13990-1-PBS)

    IHC staining of mouse brain using 13990-1-AP (same clone as 13990-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GRK2 Polyclonal antibody, PBS Only

GRK2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 13990-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

ARBK1, ADRBK1, BETA ARK1, Beta ARK 1, BARK1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

13990-1-PBS targets GRK2 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 GRK2 fusion protein Ag5071 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adrenergic, beta, receptor kinase 1
别名 ARBK1, ADRBK1, BETA ARK1, Beta ARK 1, BARK1
计算分子量 689 aa, 80 kDa
观测分子量 80 kDa
GenBank蛋白编号BC037963
基因名称 ADRBK1
Gene ID (NCBI) 156
RRIDAB_2225833
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25098
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRK2 (G-protein coupled receptor kinase 2) is also named as ADRBK1 (Beta-adrenergic receptor kinase 1), BARK, and BARK1. G-protein-coupled receptors (GPCRs) transmit extracellular signals across the cell membrane. GPCR kinases (GRKs) desensitize GPCR signals in the cell membrane. GRK2 translocation from the cytoplasm to the cell membrane in response to extracellular signals is critical to the function of the protein, where GRK phosphorylation is an important component of its translocation to the membrane.(PMID:27412951)